![]() In this article, we are going to perform trailing on NGS paired-end reads data using the GALAXY platform 2. These functions include trailing, leading, and several other quality control operations. It is a flexible tool providing several functions to be operated on reads. The thing is that i have different length reads, and in cutadapt, trimmomatic, and dada2 I saw there are options that you can say at what length of the sequence you want to trim (eg. Trimmomatic Manual: V0.32IntroductionTrimmomatic is a fast, multithreaded command line tool that can be used to trim and crop Illumina (FASTQ) data as well. Trimmomatic is a read trimming tool for Illumina NGS data 1. ![]() If i want to trim the reverse primers, is there any program (i did not see in cutadapt, trimmomatic or dada2) that you can tell to cut for example 18 bases from the end of the reads so you can trim the reverse primers just with the length and not to add the specific bases? Thanks for your time to help the newcommers!
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